Research Article

Molecular characterization of chitinase and β-1, 3-glucanase gene of soybean plant growth promoting bacterium Bacillus sp. SJ-5

Shekhar Jain1, Sarita Kumari1, Anukool Vaishnav1, Devendra Kumar Choudhary2, Kanti Prakash Sharma1*

Published 12/17/2016 .
Email: kantipsharma@rediffmail.com




Author Affiliation

Received: Oct 2016 / Accepted: Oct 2016/ Published: Dec 2016


Article History

1Department of Biosciences, College of Arts, Science & Humanities (CASH), Mody University of Science & Technology, Lakshmangarh-332311, Rajasthan, India 2Amity Institute of Microbial Technology (AIMT), Block E-3, 4th Floor, Amity University Campus, Sector-125, Noida-201313, Gautam Buddha Nagar, UP, India


How to Cite This Article

Jain, S., Kumari, S., Vaishnav, A., Choudhary, D. K., & Sharma, K. P. (2016). Molecular characterization of chitinase and +-1, 3-glucanase gene of soybean plant growth promoting bacterium Bacillus sp. SJ-5. International Journal of Agricultural and Life Sciences, 2(4), 54-59. doi: 10.9379/sf.ijals-122066-009- 0081-x.


Copyright

2016 Jain, S et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Competing Interests

The authors have declared that no competing interests exist.


Abstract

Plant-growth-promoting bacteria (PGPB) colonize the rhizosphere of many plant species and confer beneficial effects, such as increased plant growth and reduced susceptibility to diseases caused by plant pathogenic fungi. The aim of the present study was to molecularly characterize the presence of biocontrol gene chitinase and β-1, 3-glucanase of the Bacillus sp. SJ-5 to understand its role in fungal pathogen inhibition. Genomic DNA was isolated from Bacillus sp. SJ-5 and chitinase and β-1, 3-glucanase gene were amplified using specific primers. On the agarose gel 402 pb and750 bp bands were detected for chitinase and β-1, 3-glucanase respectively. Upon homology analysis it confirms the presence of chitinase and β-1, 3-glucanase gene in SJ-5 and sequences submitted to Genbank. The study indicates the potential of this PGPB to inhibit fungal pathogen through cell wall degrading enzymes production.